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瑙f瀽Protein锛¤垏G

鎻愬埌Protein A鍜孭rotein G锛岀浉淇″ぇ瀹跺苟涓嶉檶鐢燂紝鐢变簬瀹冨戝拰鎶楅珨鐨勭壒鐣版цΚ鍜屼綔鐢紝鎵浠ュ彲鐢ㄤ簬绱斿寲鎶楅珨锛屽厤鐤叡娌夋颈瀵﹂绛夛紝鍏舵噳鐢ㄩ潪甯稿唬娉涳紝鏈湡灏忕法甯跺ぇ瀹朵粩绱扮殑瑾嶈瓨涓涓婸rotein A鍜孏锛屽叐鑰呯殑鍗鍒互鍙婂浣曞緸涓伕鎿囥

1. Protein  A

20涓栫磤涓湡锛孞ensen鐧肩従鏈変竴绋泲鐧藉彲浠ヨ垏浜哄拰鍏旇娓呮姉楂斿唬娉涚祼鍚堬紝瑭茶泲鐧戒簬1964骞撮娆¤绋辩偤Protein A銆  Protein A鏄竴绋噾榛冭壊钁¤悇鐞冭弻绱拌優澹佽泲鐧借唱锛屽垎瀛愰噺42KD锛屽畠鑳藉鐗圭暟鎬у湴绲愬悎澶氱ó鍏嶇柅鐞冭泲鐧界殑Fc鍗娈佃岃垏Fab鍗鎴栬紩閸婄祼鍚堝緢寮便傚ぉ鐒剁殑Protein A鏈5鍊婭gG绲愬悎绲愭鍩熷拰鏈煡鍔熻兘鐨勯潪Fc绲愬悎鍩燂紝绲愭绀烘剰鍦栧涓嬶細

姝ょ祼妲嬬殑铔嬬櫧A澶ч噺绲愬悎IgG鐨勫悓鏅傦紝鍏堕潪Fc绲愬悎鍩熻兘绲愬悎閮ㄥ垎闆滆泲鐧斤紝灏庣坊娲楄劔涓嬩締鐨処gG绱斿害涓嶅锛屽洜姝ょ瀛稿鍒╃敤鍩哄洜宸ョ▼鐨勬柟娉曞厠闅哖rotein A鐨勫熀鍥犲苟閫茶鏀归狅紝寰楀埌閲嶇祫鍨嬭泲鐧紸锛屽叾闈炵壒鐣版х祼鍚堟槑椤檷浣庛傞噸绲勭殑铔嬬櫧A缍撻亷鏀归犲緦鏈変竴鍊婥绔殑鍗婅儽姘ㄩ吀锛屽彲浠ュ柈榛炲伓鑱埌鐡婅剛绯栦笂锛岄檷浣庣┖闁撲綅闃荤殑鍚屾檪鎻愰珮IgG鐨勭祼鍚堣兘鍔涖備竴姝ヨΚ鍜屽堡鏋愬緦妯e搧绱斿害鍙秴閬90%銆

2. Protein G

铔嬬櫧锛т締鑷簬閸婄悆鑿岋姬鏃忕殑绱拌優琛ㄩ潰铔嬬櫧锛屾槸III鍨婩c鍙楅珨锛屽垎瀛愰噺鐐25KD锛屽叾閫氶亷椤炰技浜庤泲鐧紸鐨勯潪鍏嶇柅姗熷埗鑰岀祼鍚堟姉楂旂殑Fc娈碉紝铔嬬櫧G鑸囪泲鐧紸鍧囧彲绲愬悎鎶楅珨鐨凢c娈碉紝涓嶅悓鐨勬槸锛岃泲鐧紾閭勮兘绲愬悎Fab娈碉紝涓旇泲鐧紾鍙互鏇村唬娉涚殑绲愬悎鏇村椤炲瀷鐨処gG鍒嗗瓙锛屽鍏嬮殕IgG鍒嗗瓙锛屽悓鏅傝泲鐧紾鑸囪娓呰泲鐧界祼鍚堟按骞充綆锛岀敚鐗╃磾搴﹂珮锛屼笖閰嶅熀鑴惤鏇翠綆銆傛姉楂斿熀鏈祼妲嬪拰铔嬬櫧A/G鐨勭祼鍚堜綅榛炲涓嬪湒鎵绀猴細

铔嬬櫧G鍙互绱斿寲涓嶈兘鑸囪泲鐧紸绲愬悎鐨勫摵涔冲嫊鐗╂姉楂斻傞噸绲勫瀷鐨勮泲鐧紾缍撴敼閫犲緦宸茬稉鍘婚櫎浜嗙櫧铔嬬櫧绲愬悎浣嶉粸锛堣娓呯櫧铔嬬櫧鏄姉楂斾締婧愮殑涓昏姹℃煋鐗╋級鍙婄窗鑳炶〃闈㈢祼鍚堜綅榛烇紝娓涘皯浜嗛潪鐗圭暟鎬х祼鍚堛

3. Protein A 鍜岋姬瑕拰鍔涘皪姣

铔嬬櫧A鍜岃泲鐧紾灏嶄簬涓嶅悓绋爆鍜屼笉鍚屼簽鍨嬬殑鎶楅珨绲愬悎鑳藉姏宸暟濡備笅琛ㄦ墍绀猴紝铔嬬櫧A灏嶅厰渚嗘簮鎶楅珨鐨勮Κ鍜屽姏杓冮珮锛岃泲鐧紾灏嶄簬灏忛紶绛夐儴鍒嗗摵涔冲嫊鐗╂姉楂旂殑瑕拰鍔涜純楂樸

 

鐝惧湪灏嶄簬铔嬬櫧锛″拰铔嬬櫧锛э紝鎮ㄦ槸鍚︽湁浜嗘洿娣卞叆鐨勮獚璀樺憿锛熷叾瀵︽垜鍊戦倓鏈夊绋姉楂旇Κ鍜屾潗鏂欎緵閬革紝閫傚悎浜庢崟鎹夊悇绋姉楂旂墖娈电殑Capto锛紝浠ュ強鏈夋洿濂借愬牽鎬х殑MabSelect SuRe锛屼互鍙婇珮杓夐噺楂樻ц兘鐨凪abSelect PrismA銆

 

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